Description
This assay is a quantitative sandwich enzyme immunoassay. Plates are precoated with a polyclonal antibody specific for native human MIC–1 (capture antibody). The antibody-bound MIC-1 in standards and specimens binds to a polyclonal detection antibody. An HRP conjugated anti-detection signal antibody is added followed by substrate. Color development is stopped at the appropriate time and the plates are read. Total assay time is approximately 4 hours